FastQCFastQC Report
Sun 20 Nov 2022
CO_007-2-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_007-2-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132542
Sequences flagged as poor quality0
Sequence length25-344
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA21851.6485340495842826No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG20701.5617690996061626No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC20141.5195183413559477No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG19711.4870757948423896No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC18271.3784309879132652No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG18241.3761675544355751No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG18061.3625869535694346No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC17831.3452339635738104No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT16451.2411160236000665No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG15731.1867936201355043No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG14521.095501803202004No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA14291.07814881320638No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT14231.0736219462509997No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT13641.0291077545230947No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA13341.0064734197461935No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG13301.003455508442607No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG13261.00043759713902No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG13170.9936472967059499No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT12980.9793122180139125No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA12810.9664860949736688No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG12690.9574323610629083No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT12340.931025637156524No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT12210.921217425419867No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT12190.9197084697680735No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT12070.910654735857313No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11940.900846524120656No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT11840.8933017458616891No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA11810.891038312383999No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11690.8819845784732386No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT11280.8510509876114741No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG10250.7733397715441144No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC10100.7620226041556638No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC9820.7408972250305563No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT9800.739388269378763No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT9370.706945722865205No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG9340.7046822893875149No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC9320.7031733337357216No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT9140.689592732869581No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC8890.6707307872221635No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA8890.6707307872221635No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG8620.6503598859229527No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG8580.6473419746193659No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8530.6435695854898824No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8410.634515851579122No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG7660.5779300146368699No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG7560.5703852363779028No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT7380.5568046355117623No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG7270.5485053794268987No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT7130.5379426898643449No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC7030.5303979116053779No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG6850.5168173107392373No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6640.5009732763954068No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC6420.48437476422567943No Hit
GCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTT6410.48362028639978266No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT6250.4715486411854356No Hit
GAATTTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTG6150.4640038629264686No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA5820.4391060946718776No Hit
GCAGCAGGAAGAAGAGTCACAGTTTGCTGTTTCTTCTGTCTCTGCGGTAA5600.42250758250215026No Hit
TGTTGTTGTTGGCCTTTACCAGACATTTTGCTCTCAAGCTGGTTCAATCT5470.4126993707654932No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG5380.4059090703324229No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG5360.4044001146806296No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC5350.4036456368547328No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5350.4036456368547328No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG5300.39987324772524935No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA5300.39987324772524935No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC5270.3976098142475593No Hit
GCAGCATTGTTAGCAGGATTGCGGGTGCCAATGTGATCTTTTGGTGTATT5240.39534638076986917No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5230.3945919029439725No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG5130.3870471246850055No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5000.37723891294834844No Hit
TTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACT4990.3764844351224518No Hit
GGTTCAATCTGTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTGCCAGCCATTCT4970.37497547947065835No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC4860.3666762233857947No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG4750.35837696730093105No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4740.35762248947503433No Hit
ATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTG4720.35611353382324096No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG4620.34856875556427397No Hit
CTGAGGGTCCACCAAACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGT4580.34555084426068716No Hit
GGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA4500.3395150216535136No Hit
CCAAAATATGTAATTTGCATGCATGACATAACCATCTATTTGTTCGCGTG4370.3297068099168565No Hit
GACGCATTCACATTAGTAACAAAGGCTGTCCACCATGCGAAGTGTCCCATGAGCTTATAAAGATCAGCATTCC4320.32593442078737306No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG4300.3244254651355797No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4190.31612620905071603No Hit
AGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACC4130.3115993420953358No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG4000.30179113035867877No Hit
GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCAT3930.2965097855774019No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3890.29349187427381507No Hit
GGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTC3590.2708575394969142No Hit
AAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTAT3500.26406723906384394No Hit
GCAGGTAATTGAGCAGGGTCGCCAATGTACACATAGTGCTTAGCACGTAA3450.2602948499343604No Hit
GGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACAT3360.25350454950129014No Hit
CACTAGAAATAACAACTCTGTTGTTTTCTCTAATTATAAGTCTACCTTTA3290.24822320472001327No Hit
AAAGATCAGCATTCCAAGAATGTTCTGTTATCTTTATAGCCACGGAACCT3280.2474687268941166No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC3250.24520529341642647No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG3230.24369633776463306No Hit
AATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCT3100.23388812602797604No Hit
AAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATG3060.23087021472438926No Hit
AGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAG3060.23087021472438926No Hit
CCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATA3040.22936125907259586No Hit
CAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTG3030.22860678124669914No Hit
GCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAG2950.22257095863952558No Hit
ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTA2910.2195530473359388No Hit
GCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACC2900.21879856951004212No Hit
ACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGAT2810.21200826907697184No Hit
GTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGA2750.20748140212159166No Hit
ACTACATTTATCTATAGGCAAATATTTTAATGCCTTCTCACATAGTGCAT2660.2006911016885214No Hit
GAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAATGTTACACACTGATTAA2620.19767319038493458No Hit
GATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTGACAAGTTT2620.19767319038493458No Hit
CCAAGAGCTAGCTTTTGTTGTATAAACCCACAAATGTAAGTGAAAAAACC2600.19616423473314118No Hit
CGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAAC2580.19465527908134778No Hit
ACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACA2530.1908828899518643No Hit
TGAATCTGCATCAGAGACAAAGTCATTAAGATCTGAATCGACAAGCAGCG2510.1893739343000709No Hit
CAGAACCAGCACCAAAATGTATAACTCTCATATTATAGGGTACAGCTAAT2490.18786497864827753No Hit
CAGGCAATGCATTTACAGTACAAAAGACATACTGTTCTAATGTTGAATTC2480.18711050082238082No Hit
GCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAA2480.18711050082238082No Hit
AAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACA2450.18484706734469075No Hit
CTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAA2410.18182915604110395No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG2290.17277542213034358No Hit
AGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCA2250.1697575108267568No Hit
ACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACA2220.1674940773490667No Hit
GCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCATTGAGAAAT2220.1674940773490667No Hit
ACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCT2210.16673959952317002No Hit
ACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCT2190.16523064387137662No Hit
GGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGC2180.1644761660454799No Hit
GAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACC2150.16221273256778984No Hit
CCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGG2100.15844034343830635No Hit
AGCAACACCCGGTTGCCACGCTTGACTAGATTGTAATTTTGGGTAAAATG2090.15768586561240966No Hit
GCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACT1990.15014108735344267No Hit
AGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAG1980.14938660952754598No Hit
GAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAA1930.14561422039806252No Hit
ATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGT1890.1425963090944757No Hit
GAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGAC1860.14033287561678565No Hit
CACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAA1820.13731496431319884No Hit
CGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGG1790.13505153083550875No Hit
TCCCGAAGGTGTGACTTCCATGCCAATGCGCGACATTCCGAAGAACGCTG1740.13127914170602525No Hit
GCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGAT1730.13052466388012857No Hit
AAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGA1650.124488841272955No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1600.1207164521434715No Hit
GAAGCGCTGGGGGCAAATTGTGCAATTTGCGGCCAATGTTTGTAATCAGT1540.11618958518809133No Hit
TTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGA1520.11468062953629793No Hit
GTACAAGCAAAGCAATATTGTCACTGCTACTGGAATGGTCTGTGTTTAAT1490.11241719605860784No Hit
AGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATC1470.11090824040681443No Hit
AACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACA1450.10939928475502106No Hit
GTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGC1410.10638137345143427No Hit
AACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAA1400.10562689562553756No Hit
GCAGCAAAACCTGAGTCACCTGCTACACGCTGCGAAGCTCCCAATTTGTA1390.10487241779964086No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG1370.10336346214784746No Hit
CCTTAAAGAAACCCTTAGACACAGCAAAGTCATAGAAGTCTTTGTTAAAA1350.10185450649605408No Hit
GGGTGGTTTATGTGATTTACAATAATAGCTCATACCTCCTAAGTAAAGTT1350.10185450649605408No Hit
GGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGATATG1330.1003455508442607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)